Madras Biotech
DNA & Protein Markers, Biotech Kits
Madras BioTech (MBT) is a new biotechnology company promoted by women entrepreneurs striving to develop indigenous products at affordable prices, for life science researchers in India.
In addition to DNA and protein molecular weight standards for use in everyday electrophoresis, our first products already in the market, customized services including markers of specified sizes, DNA preparations, recombinant protein overexpression and purification, etc are offered.
- Ready-to-use DNA molecular size markers
- Lambda DNA and Digests, Plasmid Vectors
- Ready-to-use Protein Molecular Weight Standards
- Biotechnology Kits
Ready-to-use DNA Molecular Size Markers
MBT's DNA markers come in four useful ranges to cover most of the requirements of researchers and students. The markers are provided in a ready-to-use format for convenience and high reproducibility. We recommend a volume of 10 µL (~1 µg total DNA) per load to ensure that distinct, prominent DNA bands are observed even with a less sensitive UV transilluminator or in a less than optimal dark chamber. Users might be able to load a volume less than that recommended (without compromising results) if a high-sensitivity gel documentation system is available.The markers have been carefully prepared under sterile conditions and stored in 10 mM Tris-HCl, pH 7.5, 10 mM EDTA and 1 µg/µl sodium azide. The stocks will be stable for several months if properly stored frozen or even refrigerated. An occasional brief exposure to room temperature should not affect the markers significantly.
The DNA markers are packaged in two convenient quantities, 150 and 250 µL, recommended for 15 and 25 loads respectively. For larger requirements, MBT will be glad to provide customized packages at special discounted prices.
| Description | Cat# | Quantity (µl) | Gel Pic |
|---|---|---|---|
| Lo-range marker: 0.1 – 1 kb This marker range has been designed to assist in determining the size of PCR product, insert and restriction analysis. Size range: 103 to 1,074 bp |
DM-1-10-S | 150 |  1.2% w/v Agarose Gel |
| DM-1-10-L | 250 | ||
| Mid-range marker: 0.5 - 5 kb This marker range should facilitate the analysis of inserts cloned into commonly used plasmids. Size range: 448 to 5,024 bp |
DM-5-50-S | 150 |  1.2% w/v Agarose Gel |
| DM-5-50-L | 250 | ||
| Hi-range marker: 2.0 - 25 kb This marker range is useful for experiments involving mega plasmids or genomic analysis. Size range: 2.0 to 25 kb |
DM-20-250-S | 150 |  0.8% w/v Agarose Gel |
| DM-20-250-L | 250 | ||
| Wide-range marker: 0.1 - 5 kb This marker contains 18 bands of a wide range that is useful for most DNA fragment sizing analyses, including PCR, plasmid and restriction analyses. Size range: 103 to 5,024 bp |
DM-1-50-S | 150 |  1.2% w/v Agarose Gel |
| DM-1-50-L | 250 |
Lambda DNA and Digests, Plasmid Vectors
MBT's other DNA products include lambda DNA, a substrate used routinely for measurement of restriction enzyme activities, and lambda DNA digested with various restriction enzymes to be used as DNA molecular weight markers.
| Description | Cat# | Quantity (µg) | |
|---|---|---|---|
| Lambda DNA | L – S | 25 |  0.8% w/v Agarose Gel |
| Lambda DNA | L – L | 50 | |
| Lambda – HindIII | LHd – S | 25 | |
| Lambda - HindIII | LHd - L | 50 | |
| Lambda - EcoRI | LERI - S | 25 | |
| Lambda - EcoRI | LERI - L | 50 | |
| pUC18 | UC18 - S | 15 | |
| pUC18 | UC18 - L | 25 | |
| pUC19 | UC19 - S | 15 | |
| pUC19 | UC19 - L | 25 | |
| pBR322 | BR322 - S | 15 | |
| pBR322 | BR322 - L | 25 |
Ready-to-use Protein Molecular Weight Standards
MBT's protein molecular weight standards cover the useful range of protein sizes that a researcher or student requires for routine experiments. Other ranges, to fulfill specialized objectives, will be introduced in the near future. The recommended loading volume is 20µL, giving approximately 5 µg of each protein sufficient for detection by Coomassie blue staining. The proteins have been dissolved and denatured in the standard SDS-PAGE sample-loading buffer. Prepared under sterile conditions, these markers have appropriate additives to ensure long-term stability when they are stored in a refrigerator or freezer and even with brief exposures to room temperature. Although boiling is not required prior to loading, doing so will not affect results. The ready-to-use marker comes in two convenient packages of 0.5 and 1 mL for 25 and 50 loads, respectively.
| Description | Cat# | Protein Marker Size Range | Quantity (µL) | |
|---|---|---|---|---|
| Mid-Range Small | PM-14-80-S | 14 - 80 kDa | 500 |  12% w/v Polyacrylamide Gel |
| Mid-Range Large | PM-14-80-L | 14 - 80 kDa | 1000 | |
| Popular-Range Small | PM-14-66-S | 14 - 66 kDa | 500 |  12% w/v Polyacrylamide Gel |
| Popular-Range Large | PM-14-66-L | 14 - 66 kDa | 1000 |
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